Mycobacterium Tuberculosis Ag85 Antibody (NA) - BSA Free

Product Discontinued

Mycobacterium Tuberculosis Ag85 Antibody (NA) - BSA Free Summary

• Immunogen: Bacterial press extract from Mycobacterium tuberculosis H37Rv
• Epitope: Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid).
• Specificity: specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:400
  • Western Blot 1:100-1:2000

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Notes

The development of this antibody and characterization has been described in the paper by Schou et al. in 1985 and further characterization was done by Andersen et al. in 1986. In the literature this antibody is being referred to as HYT 27 or IT-49; the last number was given to the antibody in connection with a WHO workshop on tuberculosis antigens, back in the 1980'es.

Alternate Names for Mycobacterium Tuberculosis Ag85 Antibody (NA) - BSA Free

• Antigen 85 complex A
• Antigen 85 complex B
• Antigen 85 complex C
• FbpA
• fbpB
• fbpC
• Fibronectin Binding protein A
• Mycolyl transferase 85A
• Secreted antigen 85 A
• Secreted antigen 85 B
• Secreted antigen 85 C
• Secreted antigen Ag85A

Background

Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.