Mycobacterium Tuberculosis 16kDa Antibody (HTM63)

• Reactivity: Ba
• Applications: WB, ELISA
• Clone: HTM63
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Mycobacterium Tuberculosis 16kDa Antibody (HTM63) Summary

• Immunogen: Recombinant full length protein: M. tuberculosis 16 kDa antigen.
• Specificity: recognises native and recombinant M. tuberculosis 16 kDa antigen.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: ELISA and Western Blot Predicted molecular weight: 16 kDa.

Reactivity Notes

Bacteria.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Background

Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.