MTT Cell Proliferation Assay Kit (Colorimetric)

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Adherent and suspension cells, Proliferating and non-proliferating cells

Order Details

MTT Cell Proliferation Assay Kit (Colorimetric) Summary

Description
Fast and sensitive MTT based assay to measure cell proliferation in response to growth factors, cytokines, mitogens and nutrients.
  • Detection method: Absorbance at 590 nm.
  • Applications: Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators that inhibit cell growth.
Kit Type
Assay Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.

Kit Components

Components
  1. MTT Reagent
  2. MTT Solvent

Background

Quantification of the number of viable cells is an indispensable tool in cell biology research. The MTT Cell Proliferation Assay Kit is a sensitive method for quantification of viable cells in proliferation and cytotoxicity assay. The method is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. Viable cells with active metabolism convert MTT into formazan, however, dead cells lose this ability. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance (590 nm) is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity, and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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