MRPS25 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC201353 and based on accession number NM_022497. The protein contains a C-terminal DDK tag.
Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), nuclear gene encoding mitochondrial protein, mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Lysate Details for MRPS25
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for MRPS25 Overexpression Lysate (Native)
- mitochondrial 28S ribosomal protein S25
- mitochondrial ribosomal protein S25
- MRP-S2528S ribosomal protein S25, mitochondrial
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. [provided by RefSeq].
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Publications for MRPS25 Lysate (NBL1-13296) (0)
There are no publications for MRPS25 Lysate (NBL1-13296).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for MRPS25 Lysate (NBL1-13296) (0)
There are no reviews for MRPS25 Lysate (NBL1-13296).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for MRPS25 Lysate (NBL1-13296) (0)
Additional MRPS25 Overexpression Lysate (Native) Products
Bioinformatics Tool for MRPS25 Lysate (NBL1-13296)
Discover related pathways, diseases and genes to MRPS25 Lysate (NBL1-13296). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on MRPS25