Mouse TH2 Cell Flow Cytometry Kit

Flow Cytometry: Mouse TH2 Cell Flow Cytometry Kit [FMC012-NOV] - For Th2 activation, mouse splenocytes were cultured for 72 hours with recombinant mouse IL-4 (5 ng/mL), anti-mouse IFN-g (10 ug/mL), anti-CD3E(10 ug/mL), and anti-CD28 (10 ug/mL) followed by a 3 hour re-stimulation with PMA/ionomycin. Cells were harvested and stained with the indicated antibodies following the procedure. Dot plots (A)show the relative IL-4 R+, IL-5+, and STAT6+ populations from activated splenocytes on CD4+-gated cells. Quadrants were set based on isotype controls. The histogram (B) shows the relative number of STAT6+ cells in unstimulated (gray line) versus stimulated (black line) splenocytes.

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Mouse TH2 Cell Flow Cytometry Kit Summary

• Description: This product is designed for the flow cytometric analysis of mouse Th2 cells using four fluorochrome-conjugated antibodies.
• Kit Type: Flow Cytometry Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.