Mouse TH1 Cell Flow Cytometry Kit

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Flow Cytometry: Mouse TH1 Cell Flow Cytometry Kit [FMC010-NOV] - For Th1 activation, mouse splenocytes were cultured for 72 hours with 5 ng/mL of recombinant mouse IL-12, 10 ug/mL of anti-IL-4, 10 ug/mL of anti-CD3, and ...read more

Product Details

Summary
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Mouse TH1 Cell Flow Cytometry Kit Summary

Description
This product is designed for the flow cytometric analysis of Th1 cells using four fluorochrome-conjugated antibodies.

Kit Type
Flow Cytometry Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 2 bottles (30 mL/bottle) of 1X Permeabilization/Wash Buffer
  2. 250 uL of Mouse IgG1-APC Isotype Control
  3. 250 uL of Mouse IgG1-PerCP Isotype Control
  4. 250 uL of Rat IgG2A-CFS Isotype Control
  5. 250 uL of Rat IgG2B-PE Isotype Control
  6. 30 mL of 1X Fixation/Permeabilization Buffer
  7. Positive Marker: 250 uL of CD4-PE Rat IgG2B; Clone GK1.5
  8. Positive Marker: 250 uL of IFN-gamma-CFS Rat IgG2A; Clone 37895
  9. Positive Marker: 250 uL of IL-12 Rbeta2-APC Mouse IgG1; Clone 305719
  10. Positive Marker:250 uL of T-bet-PerCP Mouse IgG1; Clone 525803

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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