Publications for Mouse TH1 Cell Kit (FMC010-NOV) (0)
There are no publications for Mouse TH1 Cell Kit (FMC010-NOV).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Mouse TH1 Cell Kit (FMC010-NOV) (0)
There are no reviews for Mouse TH1 Cell Kit (FMC010-NOV).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Mouse TH1 Cell Kit (FMC010-NOV) (0)
![Flow Cytometry: Mouse TH1 Cell Flow Cytometry Kit [FMC010-NOV] - For Th1 activation, mouse splenocytes were cultured for 72 hours with 5 ng/mL of recombinant mouse IL-12, 10 ug/mL of anti-IL-4, 10 ug/mL of anti-CD3, and 10 ug/mL of anti-CD28 followed by 3 hours of re-stimulation with PMA-ionomycin and 3 uM of monensin. Cells were harvested and stained with the indicated antibodies following the procedure. Dot plots show the relative T-bet+, IFN-g+, and IL-12 RB+ populations from inactivated (A) and activated (B) CD4+-gated splenocytes. Quadrants were set based on isotype controls](http://images.novusbio.com/fullsize/Mouse-TH1-Cell-Flow-Cytometry-Kit-Flow-Cytometry-FMC010-NOV-img0002.jpg "Flow Cytometry: Mouse TH1 Cell Flow Cytometry Kit [FMC010-NOV] - For Th1 activation, mouse splenocytes were cultured for 72 hours with 5 ng/mL of recombinant mouse IL-12, 10 ug/mL of anti-IL-4, 10 ug/mL of anti-CD3, and 10 ug/mL of anti-CD28 followed by 3 hours of re-stimulation with PMA-ionomycin and 3 uM of monensin. Cells were harvested and stained with the indicated antibodies following the procedure. Dot plots show the relative T-bet+, IFN-g+, and IL-12 RB+ populations from inactivated (A) and activated (B) CD4+-gated splenocytes. Quadrants were set based on isotype controls")
