Mouse Naive CD4+ T Cell Isolation Kit

Images

 
Flow Cytometry: Mouse Naive CD4+ T Cell Isolation Kit [MAGM205-NOV] - Mouse splenocytes before (A) and after (B) isolation of Naive CD4+ T cells using the MagCellect . Dot plots reflect double staining of all viable ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Kit Type
Isolation Kit

Order Details

Mouse Naive CD4+ T Cell Isolation Kit Summary

Description
The MagCellect Mouse Naive CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with naive CD4+ T cells (CD3+/CD4+/CD62L+/CD44low). Typical recoveries range from 5-35% and the purity of recovered cells ranges from 85-95%

Features
  • Yields a highly pure (85-95%) population of mouse naive CD4+ T cells
  • Isolated naive CD4+ T cells are untouched by beads or antibodies
  • Cost effective for the processing of multiple samples in combination with Magnet (Catalog #MAG997-NOV)
  • Negative selection reduces sources of experimental variation

Kit Type
Isolation Kit
Gene
CD4

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml of a Mouse Naive CD4+ T Cell Biotinylated Antibody Cocktail
  2. 1.25 ml of a Streptavidin Ferrofluid
  3. 10 ml of a 10X Buffer

Background

Mouse CD4+ T cells develop in the thymus and are released as naive T cells that also express CD62L and low levels of CD44. These cells differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental cues and the activation of specific transcription factors. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, Th17, Th9, Th22, and regulatory T cells. CD4+ T cell subsets secrete characteristic combinations of cytokines which enables them to exert diverse functions including the recruitment and activation of additional immune cells, the dampening of ongoing immune responses, and the maintenance of immunologic memory. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV) (0)

There are no publications for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV) (0)

There are no reviews for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Mouse Naive CD4+ T Cell Products

Array MAGM205-NOV

Bioinformatics Tool for Mouse Naive CD4+ T Cell Kit (MAGM205-NOV)

Discover related pathways, diseases and genes to Mouse Naive CD4+ T Cell Kit (MAGM205-NOV). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Mouse Naive CD4+ T Cell

There are no specific blogs for Mouse Naive CD4+ T Cell, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Mouse Naive CD4+ T Cell Isolation Kit and receive a gift card or discount.

Bioinformatics

Gene Symbol CD4