Mouse IgG Antibody Pair Summary
| Description |
Solid Phase sandwich ELISA for the quantitative determination of Mouse IgM using secondary antibodies for detection and capture. |
| Standard Curve Range |
625-40000 pg/mL (example only; lot dependent) |
| Sensitivity |
625 pg/mL (example only; lot dependent) |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
100 ul |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Rabbit Monoclonal anti-Mouse IgM Capture Secondary Antibody
- Rabbit Polyclonal anti-Mouse IgM Detection Secondary Antibody (HRP-conjugated)
- Standard
|
Background
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10E11 variants. Mouse IgG is one of the most common secondary antibodies used by researchers.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Mouse IgG Antibody Pair (NBP3-48426). (Showing 1 - 1 of 1 FAQ).
-
Hello, I wonder if this product for flow cytometry needs a secondary antibody or not? If yes, how to choose the secondary?
- This product is sold unconjugated and would need a secondary antibody. You will want to select a mouse IgG secondary to use with this primary. You can view all of our mouse IgG secondaries. You can filter this list down by selecting which conjugate you would prefer and which protein/target would work best for your experiment.
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