Mouse Erythrocyte Lysing Flow Cytometry Kit

Product Discontinued

Mouse Erythrocyte Lysing Flow Cytometry Kit Summary

• Description: The Mouse Erythrocyte Lysing Kit is designed to gently lyse erythrocytes from murine spleen cell preparations and stabilize the cells with fixative for later analysis by flow cytometry. Cells exposed to the lysing reagent retain their natural light scattering characteristics and fluorescent staining by flow cytometry. This erythrocyte lysing method also has an advantage over other lysing methods in that the viability of unfixed cells is maintained. The fixative and lysing reagents are provided as separate reagents, thus, allowing cells to be used in tissue culture following RBC lysis.
  
  Features
  • Gently lyses erythrocytes
  • Stabilizes leukocytes for analysis by flow cytometry
  • Maintains natural light scattering and fluorescent staining properties of leukocytes
  • Maintains leukocyte viability for subsequent cell culture manipulation
• Kit Type: Flow Cytometry Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Background

The lysis of erythrocytes from whole blood is an important initial step in the isolation and analysis of enriched leukocyte preparations. Recovered immune cells can be accurately characterized following red blood cell removal. Lysis of erythrocytes under conditions that do not disrupt lymphocytes or myeloid cells is critical for downstream applications utilizing leukocytes harvested from whole blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.