Mouse CD4+ T Cell, Small Column Enrichment Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Kit Type
Enrichment Kit

Order Details

Mouse CD4+ T Cell, Small Column Enrichment Kit Summary

Description
Designed to prepare sub-populations of CD4 mouse T cells via high affinity negative selection. The resulting column eluate is a highly enriched T cell subset population with minimal depletion of the selected T cells.

Features
  • Yields a highly pure (84-91%) population of CD4+ T cells
  • Separates cells based on gravitational flow
  • Negative selection reduces sources of experimental variation

Kit Type
Enrichment Kit
Gene
CD4

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml vials of a Monoclonal Antibody Cocktail
  2. 30 ml of a 10X Column Buffer Concentrate
  3. CD4 Subset Columns

Background

CD4+ T cells develop in the thymus and differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental cues and the activation of specific transcription factors. The isolation of CD4+ T cells is an important step in the preparation of multiple subsets including Th1, Th2, Th17, Th9, Th22, and regulatory T cells. CD4+ T cell subsets secrete characteristic combinations of cytokines which enables them to exert diverse functions including the recruitment and activation of additional immune cells, the dampening ongoing immune responses, and the maintenance of immunologic memory.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CD4