Mouse CD4+CD25+ Regulatory T Cell Isolation Kit

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Flow Cytometry: Mouse CD4+CD25+ Regulatory T Cell Isolation Kit [MAGM208-NOV] - Mouse splenocytes were prepared as described in the Cell Preparation Section. Cells were stained with Rat Anti-Mouse CD4-FITC Monoclonal ...read more
Flow Cytometry: Mouse CD4+CD25+ Regulatory T Cell Isolation Kit [MAGM208-NOV] - In the first step, CD4+ T cells were isolated from splenocytes. In the second step, CD4+CD25+ T cells were isolated from the CD4+ cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Kit Type
Isolation Kit

Order Details

Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Summary

Description
The MagCellect Mouse CD4+CD25+ Regulatory T Cell Isolation Kit is designed to isolate mouse CD4+CD25+ regulatory T cells from a preparation of splenocytes using a two-step procedure that combines both negative and positive selection techniques. The resulting cell preparation is highly enriched with CD4+CD25+ T cells with a purity of recovered cells >60%.

Features
  • Yields highly enriched populations (84-94%) of Treg cells
  • Does not require specialized instrumentation
  • Obtains isolated Treg cells by a combination of negative and positive selection

Kit Type
Isolation Kit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml of a Mouse CD4+ T Cell Biotinylated Antibody Cocktail
  2. 1.5 ml of a Streptavidin Ferrofluid
  3. 0.5 ml of a Anti-Mouse CD25 Biotinylated Antibody
  4. 25 ml of a 10X Buffer

Background

Regulatory T cells (Treg), are a minor subset of CD4+ T cells that can suppress the development and progression of immune reactions. They also play an important role in the balance between tolerance and autoimmunity. Treg cells are characterized by the expression of CD4 and CD25 on the cell surface and the intracellular transcription factor FoxP3. The isolation of regulatory T cells facilitates research into their critical role in immune system regulation. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect(TM) Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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