Mouse CD3+ T Cell, Small Column Enrichment Kit Summary
Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations. CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 45% and 65% of initial CD3+ cells loaded. The purity of recovered cells ranges from 81% to 88%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry. Designed to prepare purified murine T cell populations via high affinity negative selection. The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents. Key Benefits: Available for human, mouse, and rat cellsPurifies CD3+ T cells to 81 - 88%Based upon gravitational flowSimple procedureNo specialized equipment required
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
- Murine T Cell Selection Columns, 10X Column Buffer Concentrate
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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