Mouse CD3+ T Cell Enrichment Columns, Mini

Product Discontinued

Mouse CD3+ T Cell Enrichment Columns, Mini Summary

• Description: Principle of Selection:
  Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns. B cells bind via F(ab)-surface Immunoglobulin (Ig) interactions to glass beads coated with anti-Ig, while monocytes bind via Fc interactions to the glass beads coated with Ig. The resulting column eluate contains highly enriched T cell populations. Total CD3+ cell recovery from all available CD3+ cells loaded range between 43% and 51% and the purity of recovered cells ranges between 83% and 90%. These enriched T cell populations are then available for tissue culture, activation studies, tissue typing, immune status monitoring, and flow cytometry.

Applications/Dilutions

• Application Notes: R&D Systems' Mouse T Cell Enrichment Columns are designed to prepare purified mouse T cell populations via high affinity negative selection. The resulting column eluate is a highly enriched T cell population with minimal depletion of T cell constituents.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.