Mouse Monocyte Chemotactic Protein 5 ELISA Kit (Colorimetric)

ELISA: Mouse Monocyte Chemotactic Protein 5 ELISA Kit (Colorimetric) [NBP2-60096] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse Monocyte Chemotactic Protein 5 ELISA Kit (Colorimetric) [NBP2-60096] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 15.6 pg/mL - 1000 pg/mL
• Sensitivity: 16.82 pg/mL

Mouse Monocyte Chemotactic Protein 5 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse MCP-5. No significant cross-reactivity or interference between mouse MCP-5 and analogues was observed.
• Standard Curve Range: 15.6 pg/mL - 1000 pg/mL
• Sensitivity: 16.82 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP-5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Monocyte Chemotactic Protein 5 ELISA Kit (NBP2-60096). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.