M.O.M.® (Mouse-On-Mouse) IgG Blocking Reagent

• Reactivity: Mu
• Applications: ICC/IF, IHC, WB

M.O.M.® (Mouse-On-Mouse) IgG Blocking Reagent Summary

• Description: M.O.M.(R) (Mouse-On-Mouse) IgG Blocking Reagentis used to block endogenous mouse antibody in the tissue section and is meant as a replacement unit of M.O.M.(R) Kits (catalog numbers PK-2200-NB, FMK-2201-NB, BMK-2202-NB).

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended dilution: 2 drops per 2.5 mL of PBS or TBS. The concentration may be increased by using 3-4 drops in 2.5 mL PBS or decreased by using 2 drops in 5 mL PBS. This is the same reagent contained in the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kits (catalog numbers PK-2200-NB, FMK-2201-NB, BMK-2202-NB)..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector(R) M.O.M.(R) (Mouse-On-Mouse) System. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.