Horse anti-Mouse M.O.M.® (Mouse-On-Mouse) IgG Secondary Antibody [Biotin]

• Reactivity: Mu
• Applications: ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Horse
• Conjugate: Biotin

Horse anti-Mouse M.O.M.® (Mouse-On-Mouse) IgG Secondary Antibody [Biotin] Summary

• Description: This unit of Horse anti-Mouse, M.O.M.(R) (Mouse-On-Mouse) IgG Secondary Antibody [Biotin] has been optimized for use with the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit components. This is the same reagent contained in the following Vector(R) M.O.M.(R) Kits and is meant as a replacement unit:
  
  PK-2200-NB
  
  FMK-2201-NB
  
  BMK-2202-NB
• Immunogen: Mouse IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Horse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This reagent is supplied as 0.1 mL stock solution sufficient to make 25 mL of working solution..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are specifically designed to reduce endogenous mouse IgG staining when using mouse primary antibodies on mouse tissue. The inability of an anti-mouse secondary antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using a Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit. The result is clear, crisp specific staining of the antigens of interest. Excellent staining results for a once difficult application have now become routine with the Vector(R) M.O.M.(R) (Mouse-On-Mouse) System. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.