M.O.M.® (Mouse-On-Mouse) Basic Immunodetection Kit

• Applications: IHC, ICC/IF

M.O.M.® (Mouse-On-Mouse) Basic Immunodetection Kit Summary

• Description: The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Basic Immunodetection Kit contains a proprietary Mouse IgG Blocking Reagent and an M.O.M.(R). Biotinylated Anti-Mouse IgG reagent and is Based on the simple and easy to follow avidin or streptavidin/biotin reagent protocols. An enzyme or fluorochrome conjugate is not included allowing for a choice of any avidin or streptavidin-based detection system. Choose any peroxidase or alkaline phosphatase conjugated avidin or streptavidin for chromogenic detection, or choose a fluorochrome-labeled avidin or streptavidin.
  The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kits are useful in any mouse-on-mouse application, such as studies in genetically engineered mice, including transgenic and knock-out models, as well as mouse xenografts. The M.O.M.(R) Kits can be also used in multiple antigen staining protocols to localize two different mouse primary antibodies on the same mouse tissue sections using fluorescent or enzyme-based detection systems.
• Kit Type: Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: The M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit contains enough stock reagents to produce about 25 ml of working solution which is generally sufficient to stain approximately 250 tissue sections..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit is designed specifically to localize mouse primary antibodies on mouse tissues. The major problem with using mouse primary antibodies on mouse tissues is the inability of the anti-mouse secondary antibody to distinguish between the mouse primary antibody and endogenous mouse immunoglobulins in the tissue. This can result in high background staining which obscures specific staining. The background staining caused by the presence of endogenous mouse IgG can be essentially eliminated by using the Vector(R) M.O.M.(R) (Mouse-On-Mouse) Immunodetection Kit. The M.O.M.(R) (Mouse-On-Mouse) Kit utilizes a novel blocking agent and a special detection methodology to significantly reduce this undesired background staining. The Vector(R) M.O.M.(R) (Mouse-On-Mouse) Kit can be used with normal and genetically engineered mouse models, including transgenic, xenograft, knock out and other mutant strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.