Human, Mouse, Rat Mix-and-Match Pathway Assay ELISA Kit

Product Discontinued

Human, Mouse, Rat Mix-and-Match Pathway Assay ELISA Kit Summary

• Specificity: The Novus Mix-and-Match Pathway Assay Kit is designed to simultaneously run assays for multiple kinases on a single plate.
• Kit Type: ELISA Kit

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24112408) For the qualitative detection of differences in the levels of active phospho-proteins in key cell signaling pathways in tissue lysates, cell lysates and recombinant protein preparations. Novus Pathway Assay kits provide a quick, easy, more sensitive, and qualitatively better alternative to western blotting for kinase assay readouts. The flexibility of the Mix and Match multi-kinase ELISA array option gives researchers more of the data that they want, faster and better than any single analyte competitor system available today. Simply choose 6 of the 8 analytes below in any combination. Each selection consists of 1 pair of 8-well Antibody-Coated Microwell strips (16 tests), together with its complementary Detection Antibody. 1. p-p38 alpha (T180/Y182) 2. p-ERK2 (T183/Y185)/p-ERK1 (T202/Y204) 3. pCREB (S133) 4. p-AKT1 (S473) 5. p-AKT2 (S474) 6. Beta-Catenin (DPS33/37/T41) 7. p-GSK alpha (S21) 8. p-GSK Beta (S9) href="http://www.novusbio.com/Pathway-Assay-Kits" target="_blank">View All Available Pathway Assay Kits

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Background

Multi-kinase arrays are an alternative analysis platform to traditional Western blots. Novus' Pathway Assay Kits allow for the simultaneous detection of activation states of multiple kinases in a signal pathway on a single multiwell platform. These kits are easier and faster than traditional Western blots (2.5 hour protocol), demonstrate a significantly greater dynamic range and sensitivity, operate with much less sample (5-20 micrograms total protein), and are more cost-effective with up to six data points per assay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Mix-and-Match Pathway Assay ELISA Kit (NBP1-71668)(2)

Publications using NBP1-71668

• Brezovakova V, Sykova E, Jadhav S Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau Cells 2022-01-01 [PMID: 35563735] (SDS-Page)
• Kuru S, Yoshida M, Tatsumi S et al. Immunohistochemical localization of spatacsin in alpha-synucleinopathies. Neuropathology. 2013-09-22 [PMID: 24112408] (IHC-P, Human)

Review for Mix-and-Match Pathway Assay ELISA Kit (NBP1-71668) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 12/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24112408
• Species: Human