Mitotic Cells Antibody (8B3G) [DyLight 488]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-97684G
    • Availability
      Product Discontinued

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Mitotic Cells Antibody (8B3G) [DyLight 488] Summary

Immunogen
Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a mouse immunized with a total cell lysate of the human bladder carcinoma cell line T24.
Specificity
This antibody strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide), it clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorporation with staining, which can distinguish and quantitate the four major fractions of the cell cycle.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
Application Notes
.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Mitotic Cells Products

Array NBP1-97684G

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