Mitotic Cells Antibody (8B3G) [DyLight 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
8B3G
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 488

Mitotic Cells Antibody (8B3G) [DyLight 488] Summary

Immunogen
Clone 8B3G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a total cell lysate of the human bladder carcinoma cell line T24.
Specificity
8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorporation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Mitotic Cells Antibody (NBP1-97684G) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-97684APC
DyLight 350 Labeled NBP1-97684UV
DyLight 405 Labeled NBP1-97684V
DyLight 680 Labeled NBP1-97684FR
DyLight 755 Labeled NBP1-97684IR
PE Labeled NBP1-97684PE
PerCP Labeled NBP1-97684PCP

Additional Mitotic Cells Products

Mitotic Cells NBP1-97684G

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