Mitotic Cells Antibody (8B3G) [Alexa Fluor® 750]

Product Discontinued

Mitotic Cells Antibody (8B3G) [Alexa Fluor® 750] Summary

• Immunogen: Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a mouse immunized with a total cell lysate of the human bladder carcinoma cell line T24.
• Specificity: This antibody strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide), it clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorporation with staining, which can distinguish and quantitate the four major fractions of the cell cycle.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined..

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.