Mitochondrial Apoptosis Detection Assay Kit (Fluorometric)

Product Discontinued

Mitochondrial Apoptosis Detection Assay Kit (Fluorometric) Summary

• Description: Simple method to distinguish between healthy and apoptotic cells by detecting change in the mitochondrial transmembrane potential.
  
  Detection method: Fluorescence microscopy and Flow Cytometry (Ex/Em = 488/530 nm and optional Ex/Em = 488/590 nm) Applications: Detect mitochondrial changes in apoptosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65310)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, the dye accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, the dye cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.