MHC Class I RT1Ac Antibody (OX-27) - BSA Free

Flow Cytometry: MHC Class I RT1Ac Antibody (OX-27) [NB100-64951] - Analysis using the FITC conjugate of NB100-64951. Staining of BN rat spleen cells.

Flow Cytometry: MHC Class I RT1Ac Antibody (OX-27) [NB100-64951] - Staining of BN rat spleen cells with MOUSE ANTI RAT RT1Ac (NB100-64951) visualised with MOUSE IgG:FITC (RAT ABSORBED) (STAR70).

Flow Cytometry: MHC Class I RT1Ac Antibody (OX-27) [NB100-64951] - Analysis using the FITC conjugate of NB100-64951. Staining of rat spleen cells.

• Reactivity: Rt
• Applications: Flow, IHC, IHC-Fr
• Clone: OX-27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MHC Class I RT1Ac Antibody (OX-27) - BSA Free Summary

• Immunogen: PHA activated rat lymphocytes
• Localization: Cell Membrane; Type I membrane protein.
• Specificity: NB100-64951 recognises a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for MHC Class I RT1Ac Antibody (OX-27) - BSA Free

• HLA-E

Background

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MHC Class I RT1Ac Antibody (NB100-64951)(2)

Publications using NB100-64951

• Stumbles PA, Thomas JA et al. Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity. J Exp Med. [PMID: 9841916] (FLOW, Rat)
• Crook K, Hunt SV. Enrichment of early fetal-liver hemopoietic stem cells of the rat using monoclonal antibodies against the transferrin receptor, Thy-1, and MRC-OX82. Dev Immunol. [PMID: 8924759] (FLOW, Rat)