MGC12965 Overexpression Overexpression Lysate

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Western Blot: MGC12965 Overexpression Overexpression Lysate (Adult Normal) [NBL1-13057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MGC12965 Overexpression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MGC12965 Overexpression Overexpression Lysate Summary

Description
Transient overexpression lysate of similar to Cytochrome c, somatic (MGC12965) The lysate was created in HEK293T cells, using Plasmid ID RC202962 and based on accession number NM_198519. The protein contains a C-MYC/DDK Tag.

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-13057

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