Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

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ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161] - A typical standard curve for Methotrexate ELISA kit. These standard curves are provided for demonstration only. A standard curve should be ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Human, mouse, and rat serum, plasma, urine
Standard Curve Range
0.13-1000 ng/ml
Sensitivity
0.087 ng/ml

Order Details

Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.13-1000 ng/ml
Sensitivity
0.087 ng/ml
Assay Type
Competitive ELISA
Inter-Assay
Low Concentration %CV - 22.4% High Concentration %CV - 17.1%
Intra-Assay
Low Concentration %CV - 20.5% High Concentration %CV - 9.8%
Spike Recovery
Human Serum Low - 71%
Medium - 89%
High - 86%

Human Plasma Low - 91%
Medium - 107%
High - 77%

Mouse Serum Low - 141%
Medium - 140%
High - 135%

Mouse Plasma Low - 90%
Medium - 168%
High - 56%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. and Stop Solution
  2. Antibody Diluent
  3. Antibody
  4. Assay Buffer 13
  5. Conjugate
  6. Microtiter Plate
  7. Standard
  8. Substrate
  9. Wash Buffer Concentrate

Alternate Names for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

  • MTX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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