Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161] - A typical standard curve for Methotrexate ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA
• Suitable Sample Type: Human, mouse, and rat serum, plasma, urine
• Standard Curve Range: 0.13-1000 ng/ml
• Sensitivity: 0.087 ng/ml

Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.13-1000 ng/ml
• Sensitivity: 0.087 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 22.4% High Concentration %CV - 17.1%
• Intra-Assay: Low Concentration %CV - 20.5% High Concentration %CV - 9.8%
• Spike Recovery: Human Serum Low - 71%
  Medium - 89%
  High - 86%
  
  Human Plasma Low - 91%
  Medium - 107%
  High - 77%
  
  Mouse Serum Low - 141%
  Medium - 140%
  High - 135%
  
  Mouse Plasma Low - 90%
  Medium - 168%
  High - 56%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)

• (+)-Amethopterin
• 4-amino-10-methylfolic acid
• 4-Amino-N10-methylpteroylglutamic Acid
• 59-05-2
• Amethopterine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Methotrexate ELISA Kit (NBP2-62161). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.