MES-SA Dx5 Whole Cell Lysate Summary
| Description |
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Whole cell lysate (non-denatured) |
| Localization |
Uterus |
Preparation Method |
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay. |
Applications/Dilutions
| Dilutions |
- Immunoprecipitation
- Western Blot
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF). |
| Concentration |
5 mg/ml |
Lysate Details for Array
| Type |
Cell |
| Tissue |
Uterus |
| Protein State |
Native |
| Subcellular Fraction |
Whole |
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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