![ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - Standard Reference Curve](http://images.novusbio.com/fullsize/Melatonin-ELISA-Kit-Colorimetric-ELISA-NBP2-62160-img0001.jpg "ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - Standard Reference Curve")
| Applications | ELISA |
| Suitable Sample Type | Saliva, serum, plasma, fruit samples |
| Standard Curve Range | 0.08-50 ng/ml |
| Sensitivity | 0.162 ng/ml |
| Standard Curve Range | 0.08-50 ng/ml |
| Sensitivity | 0.162 ng/ml |
| Assay Type | Competitive ELISA |
| Inter-Assay | Low Concentration %CV - 17.54%
High Concentration %CV - 7.36% |
| Intra-Assay | Low Concentration %CV - 13.74%
High Concentration %CV - 4.31% |
| Spike Recovery | Human Serum
Low - 124% Medium - 83% High - 102% Human EDTA Plasma Low - 112.2% Medium - 90% High - 101% Rat Serum Low - 112.4% Medium - 99.6% High - 89.7% Mouse Serum Low - 115.6% Medium - 105.8% |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
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![ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - To assess parallelism, human serum and EDTA plasma samples,human saliva, porcine along with other matrix samples wereserially diluted in 1X Stabilizer. The samples were then run in theassay. The melatonin concentration in each sample wasdetermined from the standard curve. Concentrations were plottedas a function of sample dilution. Parallelism of the curvesdemonstrates that the standard binding characteristics are similarenough to allow the accurate determination of native melatoninlevels in diluted samples matrices of animal and plant origin.](http://images.novusbio.com/fullsize/Melatonin-ELISA-Kit-Colorimetric-ELISA-NBP2-62160-img0002.jpg "ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - To assess parallelism, human serum and EDTA plasma samples,human saliva, porcine along with other matrix samples wereserially diluted in 1X Stabilizer. The samples were then run in theassay. The melatonin concentration in each sample wasdetermined from the standard curve. Concentrations were plottedas a function of sample dilution. Parallelism of the curvesdemonstrates that the standard binding characteristics are similarenough to allow the accurate determination of native melatoninlevels in diluted samples matrices of animal and plant origin.")