Melatonin ELISA Kit (Colorimetric)

ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - Standard Reference Curve

ELISA: Melatonin ELISA Kit (Colorimetric) [NBP2-62160] - To assess parallelism, human serum and EDTA plasma samples,human saliva, porcine along with other matrix samples wereserially diluted in 1X Stabilizer. The samples were then run in theassay. The melatonin concentration in each sample wasdetermined from the standard curve. Concentrations were plottedas a function of sample dilution. Parallelism of the curvesdemonstrates that the standard binding characteristics are similarenough to allow the accurate determination of native melatoninlevels in diluted samples matrices of animal and plant origin.

• Applications: ELISA
• Suitable Sample Type: Saliva, serum, plasma, fruit samples
• Standard Curve Range: 0.08-50 ng/ml
• Sensitivity: 0.162 ng/ml

Melatonin ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.08-50 ng/ml
• Sensitivity: 0.162 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 17.54% High Concentration %CV - 7.36%
• Intra-Assay: Low Concentration %CV - 13.74% High Concentration %CV - 4.31%
• Spike Recovery: Human Serum Low - 124%
  Medium - 83%
  High - 102%
  
  Human EDTA Plasma Low - 112.2%
  Medium - 90%
  High - 101%
  
  Rat Serum Low - 112.4%
  Medium - 99.6%
  High - 89.7%
  
  Mouse Serum Low - 115.6%
  Medium - 105.8%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Melatonin ELISA Kit (Colorimetric)

• Melatonin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Melatonin ELISA Kit (NBP2-62160)(4)

Publications using NBP2-62160

• Khan J, Elsharkawy E, Fu Y et al. Melatonin alleviates lead-induced stress in rice through physiological regulation and molecular defense mechanisms. Scientific reports 2025-10-06 [PMID: 41053258]
• Baojing Zhao, Zhen Yu, Junjie Sun, Weisheng Cheng, Tao Yu, Yuanyuan Yang, Zhaolian Wei, Zongzhi Yin Light pollution during pregnancy influences the growth of offspring in rats. Ecotoxicology and environmental safety 2024-05-23 [PMID: 38788564]
• Kennaway DJ Can we believe results obtained from plasma melatonin ELISA kits? Chronobiology international 2021-03-04 [PMID: 33663310]
• David J Kennaway Measuring melatonin by immunoassay. Journal of pineal research 2021-08-19 [PMID: 32281677]

FAQs for Melatonin ELISA Kit (NBP2-62160). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.