MCF-7 Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2 mg/ml

Order Details

MCF-7 Whole Cell Lysate Summary

Specificity
MCF7 (Human Breast Adenocarcinoma)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
Buffer
SDS sample buffer containing 50 mM DTT.
Concentration
2 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Breast
Tissue Condition
Adenocarcinoma
Subcellular Fraction
Whole

Notes

GROWTH MEDIA: ATCC medium (Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS containing 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, and 10% FBS)

Background

MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBP2-03964

Additional MCF-7 Products

Array NBP2-03964

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