MCF-7 Whole Cell Lysate Summary
MCF7 (Human Breast Adenocarcinoma)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
SDS sample buffer containing 50 mM DTT.
Lysate Details for Array
GROWTH MEDIA: ATCC medium (Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS containing 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids and 1.0 mM sodium pyruvate, and 10% FBS)
MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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