MCF-7 Nuclear Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2 mg/ml

Order Details

MCF-7 Nuclear Cell Lysate Summary

Description
Nuclear extract cell lysate (denatured)
Localization
mammary gland; breast
Preparation
Method
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A and then centrifugated at 25000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged divided into aliquots and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2.5 mg/ml and then mixed with 5X Sample Buffer to become final 2 mg/ml in Sample Buffer. The lysate was heated at 95C for 5 min and cooled rapidly.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
-2146826273
Concentration
2 mg/ml

Lysate Details for MCF-7

Type
Cell
Tissue
Breast
Protein State
Denatured
Subcellular Fraction
Nuclear

Notes

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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