Maltose Binding Protein Antibody (R29.6) - Azide and BSA Free

Western Blot: Maltose Binding Protein Antibody (R29.6) - Azide and BSA Free [NBP3-08444] - Western Blot Analysis of MBP-Tag Recombinant protein using Maltose Binding Protein Mouse Monoclonal Antibody (R29.6).

• Reactivity: Ec
• Applications: WB, ICC/IF
• Clone: R29.6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Maltose Binding Protein Antibody (R29.6) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07585).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: MOS maltose binding protein fusion protein.
• Localization: Periplasm.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

MBP fusion proteins.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Maltose Binding Protein Antibody (R29.6) - Azide and BSA Free

• Mal E
• MalE
• Maltodextrin binding protein
• Maltose ABC transporter periplasmic protein
• Maltose binding periplasmic protein
• MBP
• MMBP
• Periplasmic maltose binding protein

Background

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.