Maleimide Activated R-Phycoerythrin

• Applications: IHC

Maleimide Activated R-Phycoerythrin Summary

• Description: Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.
  
  

  Features	Benefits
  Compatible protein thiolation kit available	No specialist knowledge required
  Covalent bond	Highly stable conjugates
  Maleimide activated labels can be used with a range of biomolecules, including oligonucleotides	Flexible
  Scalable	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Traditional, well-known conjugation chemistry	Trusted method

  
  
  

  Maleimide activated labels

  Maleimide - RPE

  Maleimide - HRP

  Maleimide - Alkaline Phosphatase

  Maleimide - APC

  Maleimide-Streptavidin

  Maleimide-Ovalbumin

  Maleimide-BSA

  Maleimide - KLH

  
  
  If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label:
  
  1. Protein Thiolation Kit
  
  Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein.
  
  Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working.
  
  2. Thiol Quantification Kit
  
  The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction.
  
  The assay is in a convenient 96-well plate format and contains all the necessary reagents required.
  
  Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals.
• Specificity: Maleimide-activated R-Phycoerythrin is designed to facilitate the conjugation of RPhycoerythrin to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The reactive maleimide group that has been added to the R-Phycoerythrin allows conjugation reactions to be performed very efficiently at physiological pH.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Supplied as freeze dried formulations

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Maledimide and periodate activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) or amine (-NH2) group respectively. The reactive maleimide or periodate groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

Innova Biosciences activated labels are supplied as stable freeze dried formulations, with all material being rigorously QC tested using thiolated biomolecules to ensure high conjugation efficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Maleimide Activated R-Phycoerythrin (403-0002)(1)

Publication using 403-0002

• Trilling AK, Harmsen MM, Ruigrok VJ et al. The effect of uniform capture molecule orientation on biosensor sensitivity: dependence on analyte properties. Biosens Bioelectron. 2012-01-01 [PMID: 22878083]