Maleimide Activated KLH

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Maleimide Activated KLH Summary

Description
Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) group. The reactive maleimide groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

FeaturesBenefits
Compatible protein thiolation kit availableNo specialist knowledge required
Covalent bondHighly stable conjugates
Maleimide activated labels can be used with a range of biomolecules, including oligonucleotidesFlexible
ScalableEasy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Traditional, well-known conjugation chemistryTrusted method


Maleimide activated labels
Maleimide - RPE
Maleimide - HRP
Maleimide - Alkaline Phosphatase
Maleimide - APC
Maleimide-Streptavidin
Maleimide-Ovalbumin
Maleimide-BSA
Maleimide - KLH


If the biomolecule does not have a free thiol group, it will require activation to generate a free thiol group prior to conjugation with the maleimide label:

1. Protein Thiolation Kit

Designed to facilitate the conjugation of proteins to our maleimide-activated labels. Simply add your protein solution to the lyophilized thiolation reagents to activate your protein. These kits are designed to activate 2mg or 5mg of your protein.

Each thiolation kit is also supplied with a thiolation detection kit provided as a positive test for protein thiolation, giving you the option to confirm the chemistry is working.

2. Thiol Quantification Kit

The thiol quantification assay kit allows you to quantify the amount of thiol/sulfhydryl (-SH) groups in samples of thiolated proteins or antibodies. Quantifying the number of free thiols in a sample can be useful in a number of applications and allows for a more controlled conjugation reaction.

The assay is in a convenient 96-well plate format and contains all the necessary reagents required.
Specificity
Maleimide-activated KLH is designed to facilitate the conjugation of the KLH (Keyhole Limpet Hemocyanin) to proteins, peptides and ligands that contain sulfhydryl (-SH) groups. The added maleimide groups allow conjugation reactions to be performed very efficiently at physiological pH.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Supplied as freeze dried formulations

Notes

Molecules that do not contain -SH groups can be chemically modified to introduce the required thiol functionality. In the case of antibodies, disulfide (S-S) bridges may also be cleaved to release free thiols.

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Background

Maledimide and periodate activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (-SH) or amine (-NH2) group respectively. The reactive maleimide or periodate groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance.

Innova Biosciences activated labels are supplied as stable freeze dried formulations, with all material being rigorously QC tested using thiolated biomolecules to ensure high conjugation efficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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