Malate Dehydrogenase Activity Assay Kit (Fluorometric)

Malate Dehydrogenase Activity Fluorometric Assay Kit [NBP2-59750]

Malate Dehydrogenase Activity Fluorometric Assay Kit [NBP2-59750]

Malate Dehydrogenase Activity Fluorometric Assay Kit [NBP2-59750]

Product Discontinued

Malate Dehydrogenase Activity Assay Kit (Fluorometric) Summary

• Description: This kit provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with the Probe forming a fluorophore. Detection method: Fluorescence (Ex/Em = 535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 uU of MDH activity.
  
  
  Suitable for the analysis of citric acid cycle and malate-asparate shuttle Can be used with animal heart, liver, muscle tissues Can be used with purified mitochondria Can be used with adherent or suspension cell cultures
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab235593)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.

Background

Malate Dehydrogenase (MDH) (EC 1.1.1.37) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer's disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.