MagCellect™ Plus Human EpCAM+ Cell Isolation Kit

Product Discontinued

MagCellect™ Plus Human EpCAM+ Cell Isolation Kit Summary

• Description: A mononuclear cell suspension is first incubated with the Human EpCAM Biotinylated Antibody that targets the desired cells. MagCellect(TM) Streptavidin (SA) Ferrofluid is next added to the reaction that allows the SA coated nanoparticles to interact with the cells tagged with the monoclonal antibodies. The tube containing the cell suspension is then placed within a magnetic field. Magnetically tagged cells will migrate toward the magnet (desired cell fraction), leaving the untagged cells (unwanted cell population) in suspension to be harvested by aspiration while the tube remains in the magnetic field. The tube containing the magnetically selected (desired) cells is then removed from the magnet and the cells are resuspended in MagCellectTM Plus Buffer or tissue culture media. The enriched cell preparation is then available for a variety of applications including tissue culture, immune status monitoring and flow cytometry
• Kit Type: Isolation Kit
• Gene: EPCAM

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as TROP 1, is a calcium-dependent homophilic adhesion protein. It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1. During embryonic development, EpCAM is expressed in lung, kidney, liver, pancreas, skin, and germ cells. In the adult, it is expressed on basolateral cell membranes of simple, pseudo-stratified, and transitional epithelia. EpCAM is upregulated in proliferating epithelial tissues, during adult liver regeneration, and many carcinomas. MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect( Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: EPCAM