Lymphocytes Antibody (MsGp2)

Product Discontinued

Lymphocytes Antibody (MsGp2) Summary

• Immunogen: Nylon wool adherent B cells
• Specificity: NB100-64632 reacts with an antigen on the majority of T and B lymphocytes and thymocytes, although the antigen is not expressed by germinal centre cells of the lymph node. MSgp2 reacts strongly with epidermal Langerhans cells, giving a superior profile than anti-MHC Class II antibodies on these cells. MSgp2 has the same distribution as monoclonal antibody CT4 which detects an antigen reportedly involved in the organ non-specific trafficing of guinea pig lymphocytes. However, unlike CT4, MSgp2 does not functionally inhibit lymphocyte adherence to high endothelial venules in vitro.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: This antibody does not work in Flow Cytometry, ELISA, Immunohistochemistry - Paraffin, Imunohistochemistry - Resin, Western Blotting, Radioimmunoassays, and Immunoprecipitation.

Reactivity Notes

Reacts with Guinea Pig.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Background

Lymphocytes have receptors for antigen and confer specificity on an immune response. Lymphocytes express receptors with varying affinity for the antigen in question. Langerhans Cells are present in the skin and process efficiently antigen but cannot present it to T cells. Langerhans cells have been shown to pick up antigen in skin and carry it via afferent lymphatic vessels to lymph nodes. Recognises an antigen expressed by Langerhans cells, intermediate dendritic cells and interdigitating cells in T cell areas of rat lymphoid organs. Monocytes and macrophages in various organs and tissues are also stained.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Lymphocytes Antibody (NB100-64632). (Showing 1 - 1 of 1 FAQ).

1. What is this product's buffer composition?
   • NB100-64632 is produced using following media constituents: RPMI 1640, Gentamicin, Kanamycin, Sodium Pyruvate, GlutaMAX 1 (stable form of L-Glutamine), Glucose, Non essential Amino Acids, and Fetal Bovine Serum (Ultra low IgG).