Ly-49C/F/I/H Antibody (14B11) [FITC]

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00194
    • Availability
      Product Discontinued

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Ly-49C/F/I/H Antibody (14B11) [FITC] Summary

Immunogen
This antibody was generated by immunizing host with IL-2-activated killer cells (LAK) from C57BL/6 mic
Localization
NK and NK-T cells, some CD8+ T cells
Isotype
IgG
Clonality
Monoclonal
Host
Golden Syrian Hamster
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The 14B11 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Ly-49C/F/I/H Antibody (14B11) [FITC]

  • 5E6
  • Cmv1
  • Killer cell lectin like receptor 3
  • Killer cell lectin like receptor 6
  • Killer cell lectin like receptor 8
  • Killer cell lectin like receptor 9
  • Klra3
  • Klra6
  • Klra8
  • Klra9
  • Ly49 C antigen
  • Ly49 F antigen
  • Ly49 H antigen
  • Ly49 I antigen
  • Lymphocyte antigen 49C
  • Lymphocyte antigen 49F
  • Lymphocyte antigen 49H
  • Lymphocyte antigen 49I
  • T cell surface glycoprotein Ly 49C
  • T cell surface glycoprotein Ly 49F
  • T cell surface glycoprotein Ly 49H
  • T cell surface glycoprotein Ly 49I

Background

The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody reacts with 3 putative inhibitory members of the Ly-49 family, F, I, and C and one putative activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It is reported that 14B11 enhances the redirected lysis of FcR+ target cells by IL-2-activated NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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