Ly-49C/F/I/H Antibody (14B11)

Product Discontinued

Ly-49C/F/I/H Antibody (14B11) Summary

• Immunogen: This antibody was generated by immunizing host with IL-2-activated killer cells (LAK) from C57BL/6 mic
• Localization: NK and NK-T cells, some CD8⁺ T cells
• Isotype: IgG
• Clonality: Monoclonal
• Host: Golden Syrian Hamster
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ly-49C/F/I/H Antibody (14B11)

• 5E6
• Cmv1
• Killer cell lectin like receptor 3
• Killer cell lectin like receptor 6
• Killer cell lectin like receptor 8
• Killer cell lectin like receptor 9
• Klra3
• Klra6
• Klra8
• Klra9
• Ly49 C antigen
• Ly49 F antigen
• Ly49 H antigen
• Ly49 I antigen
• Lymphocyte antigen 49C
• Lymphocyte antigen 49F
• Lymphocyte antigen 49H
• Lymphocyte antigen 49I
• T cell surface glycoprotein Ly 49C
• T cell surface glycoprotein Ly 49F
• T cell surface glycoprotein Ly 49H
• T cell surface glycoprotein Ly 49I

Background

The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8+ cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.