Lung Matched Tumor and Normal Tissue Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary

Order Details

Lung Matched Tumor and Normal Tissue Lysate Summary

Description
1 x Human lung tumor tissue lysate (1 mg/ml, 100 ug/vial)
1 x Human lung normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)

Matched Tumor & Normal Tissue Lysate Set

Diagnosis: Large Cell Carcinoma

Sex: Male

Age: 46

Grade: 3

Stage: III

Tumor Pathology Data


Location: Left upper lobe

Gross findings: Tumor size 6 x 5 cm. Cut section hemorrhagic and dark red. Axillary lymph node: 1

Microscopic Findings

Tumor shows malignant cells. The malignant cells are large and cuboidal or columnar. The cytoplasm is granular and vacuolated with large nucleoli. Nuclei have irregular nuclear membranes. Many monstrous nuclei and neutrophil cells. Axillary lymph node is positive.

Preparation Method

Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4

Applications/Dilutions

Application Notes
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.

Lysate Details for Array

Type
Matched Tumor and Normal Tissue
Life Stage
Adult
Tissue
Lung

Notes

The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.

This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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