Mouse Lung Whole Tissue Lysate (Adult Whole Normal)

Product Discontinued

Mouse Lung Whole Tissue Lysate (Adult Whole Normal) Summary

• Specificity: Lung

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Respiratory
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Notes

Protein supplied in 1x Laemmli Sample Buffer without 2-mercaptoethanol. 2-Mercaptoethanol should be added to 5% prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Lung Lysate (NB820-59663). (Showing 1 - 2 of 2 FAQ).

1. For NB820-59663 lysate, I'd like to know the extraction procedure. Which buffer has been used? Protease, Phosphotase Inhibitors? And how will the lysate be delivered (which buffer and concentration)?
   • A total protein extraction kit was used for the extraction of the total protein lysate. This kit uses a TM Buffer (HEPES (pH7.9), MgCl2, Kcl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate) and a cocktail of protease inhibitors. The protein is delivered at 5 ug/ul concentration. We have protease inhibitor cocktail in the lysate, but we don't release the specific protease inhibitor information.
2. Is this lysate derived from perfused tissues?
   • Unfortunately, this lysate is not from perfused tissues.