Human Lung Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Lung Whole Tissue Lysate (Adult Normal) [NB820-59239] - analysis of Beta Actin in human lung tissue lysate (20ug) using Beta Actin antibody (Cat.# NBP1-47423) followed by goat anti-mouse kappa light ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Lung Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Lung: Right Middle Lobe

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 5
using
NB820-59239 in the following application:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40 and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Respiratory
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Review for Lung Lysate (NB820-59239) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB820-59239:
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WB
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Images Ratings Applications Species Date Details
Western Blot Lung NB820-59239
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reviewed by:
Ming Lu
WB Human 10/23/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman Lung Whole Tissue Lysate (Adult Normal)
SpeciesHuman
LotA703391
CommentsHuman Lung Whole Tissue Lysate (Adult Normal) is very easy to work with. Novus is generous to give 1mg which can be used for 50 westerns.

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratiob-actin: Novusbio cat#NBP1-47423 1:1000, in blocking buffer 4C overnight

Secondary Antibody

Secondary Descriptionanti mouse light chain kappa Novus NB7549
Secondary Manufacturer Cat#Novus NB7549
Secondary Concentration1:5000

Details

Detection Notes1 min exposure gives band at correct MW.

Comments

CommentsHuman Lung Whole Tissue Lysate (Adult Normal) is very easy to work with. Novus is generous to give 1mg which can be used for 50 westerns.

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Recent Reviews

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Ming Lu
10/23/2014
Application: WB
Species: Human