LS1034 Whole Cell Lysate

Product Discontinued

LS1034 Whole Cell Lysate Summary

• Description: Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue. Whole cell lysate (non-denatured)
• Localization: Cecum
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay.

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: In modified RIPA Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
• Concentration: 5 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Digestive/Waste
• Protein State: Native
• Subcellular Fraction: Whole

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.