| Immunogen | LOVO cells (Human colorectal adenocarcinoma) were grown to 85% confluence, harvested, homogenized in modified RIPA buffer and cleared by centrifugation. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Modified RIPA buffer:
150 mM NaCl, 50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 1 mM EDTA, 1 mM PMSF, 5 ug/ml Aprotinin, 1 ug/ml Pepstatin-A, 2 ug/ml Leupeptin,
1 mM Na3VO4, 1 mM NaF |
| Concentration | 2 mg/ml |
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