LOC400657 Overexpression Lysate (Native)


Western Blot: LOC400657 Overexpression Lysate (Adult Normal) [NBL1-12601] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LOC400657.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

LOC400657 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207252 and based on accession number NM_001008234. The protein contains a C-terminal DDK tag.
Homo sapiens hypothetical gene supported by BC036588 (LOC400657), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for LOC400657

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LOC400657 Overexpression Lysate (Native)

  • FLJ10991
  • hypothetical LOC400657


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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LOC400657 NBL1-12601

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Gene Symbol LOC400657