LOC284912 Overexpression Lysate (Native)

Images

 
Western Blot: LOC284912 Overexpression Lysate (Adult Normal) [NBL1-12593] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LOC284912.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

LOC284912 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200986 and based on accession number NM_203375. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hypothetical gene supported by BC001801 (LOC284912), mRNA.
Gene
LOC284912

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for LOC284912

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for LOC284912 Overexpression Lysate (Native)

  • hypothetical LOC284912
  • MGC3170

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Additional LOC284912 Products

LOC284912 NBL1-12593

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Bioinformatics

Gene Symbol LOC284912