Human LKM-1 ELISA Kit (Colorimetric) Summary
| Description |
LKM-1 Ab ELISA Kit is a solid phase enzyme immunoassay employing human recombinant cytochrome p450 IID6 for the detection of antibodies against liver-kidney microsomes (LKM) in human serum. |
| Sensitivity |
1.0 U/mL |
| Assay Type |
solid phase enzyme-linked immunosorbent assay |
| Inter-Assay |
CV% < 2.67% |
| Intra-Assay |
CV% < 2.67% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
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| Application Notes |
LKM-1 Ab ELISA Kit is a solid phase enzyme immunoassay employing human recombinant cytochrome p450 IID6 for the quantitative and qualitative detection of antibodies against liver-kidney microsomes (LKM) in human serum. |
Packaging, Storage & Formulations
Kit Components
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Components
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- Calibrators A-F (0, 3, 10, 30, 100, 300 U/mL), containing: Human serum (diluted), BSA,sodium azide < 0.1% (preservative). Ready to use. Color increasing with concentration:yellow solutions. Ready to use.
- Conjugate IgG. Containing: Anti-human immunoglobulins conjugated to horseradishperoxidase, BSA. Ready to use.
- Cut-off Control. Containing: Human serum (diluted), BSA, sodium azide < 0.1%(preservative). Ready to use.
- Microplate: Consisting of 12 modules of 8 wells each. Ready to use.
- Negative Control. Containing: Human serum (diluted), BSA, sodium azide < 0.1%(preservative). Ready to use.
- Positive Control. Containing: Human serum (diluted), BSA, sodium azide < 0.1%(preservative). Ready to use.
- Sample buffer 5x, 5x concentrated. Containing: Tris, NaCl, BSA, sodium azide <0.1%(preservative).
- Stop solution. Containing: 1M Hydrochloric Acid. Ready to use.
- TMB Substrate. Containing Stablilized TMB/H2O2. Ready to use.
- Wash buffer 50x, 50x concentrated. Containing: Tris, NaCl, Tween 20, sodium azide <0.1% (preservative).
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Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for LKM-1 ELISA Kit (KA1280). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
