Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) [KA1329] - Inhibition of soybean 15-lipoxygenase by NDGA (IC50= 9 uM).

• Reactivity: Hu
• Applications: Func, Enzyme Activity
• Suitable Sample Type: Compounds expected to inhibit Lipoxygenases

Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) Summary

• Description: Lipoxygenase Inhibitor Screening Assay Kit is a measurement of detecting hydeoperoxides produced in the lipoxygenation reaction.
• Assay Type: Colorimetric
• Sample Volume: 10 uL/well. All samples should be assayed in duplicate at minimum.
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • Functional

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Lipoxygenases (LOs) are non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system. The initial product of this reaction is a 4-hydroperoxy cis-trans-1,3-conjugated pentadienyl moiety within the unsaturated fatty acid. The three main LO enzymes are designated 5-, 12-, and 15-LO based on the position of the introduced hydroperoxide. Linoleate and arachidonate are the common substrates for LOs in plants and animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lipoxygenase Inhibitor Screening Kit (KA1329)(8)

Publications using KA1329

• Hongwei W, Enjing C, Jiaming L et al. Design and synthesis of novel indole and indazole-piperazine pyrimidine derivatives with anti-inflammatory and neuroprotective activities for ischemic stroke treatment. Eur J Med Chem. 2022-07-21 [PMID: 35931005]
• Huang Y, Zhang B, Li J et al. Design, synthesis, biological evaluation and docking study of novel indole-2-amide as anti-inflammatory agents with dual inhibition of COX and 5-LOX. Eur J Med Chem. 2019-07-04 [PMID: 31299586]
• Afifi O, Shaaban O, Abd H et al. Synthesis and biological evaluation of purine-pyrazole hybrids incorporating thiazole, thiazolidinone or rhodanine moiety as 15-LOX inhibitors endowed with anticancer and antioxidant potential. Bioorg Chem. 2019-04-01 [PMID: 30999135]
• Araujo JS, Chambo ED, Costa MAPC et al. Chemical Composition and Biological Activities of Mono- and Heterofloral Bee Pollen of Different Geographical Origins. Int J Mol Sci 2017-04-27 [PMID: 28448467]
• Abd El Razik HA, Badr MH, Atta AH et al. Benzodioxole-Pyrazole Hybrids as Anti-Inflammatory and Analgesic Agents with COX-1,2/5-LOX Inhibition and Antioxidant Potential. Arch Pharm (Weinheim) 2017-04-18 [PMID: 28418202]
• Plackova P, Sala M, Smidkova M et al. 9-Norbornyl-6-chloropurine (NCP) induces cell death through GSH depletion-associated ER stress and mitochondrial dysfunction. Free Radic Biol Med 2016-06-07 [PMID: 27288283]
• Man Kyu Huh, Kyung-Soon Cho, Sung-Jong Jeon. et al. Inhibitory effect of lipoxygenase and DPPH radical scavening activity of fraxinus rhynchophylla. European Journal of Advanced Research in Biological and Life Sciences 2015-07-01
• Chaaban I, Rizk OH, Ibrahim TM et al. Synthesis, anti-inflammatory screening, molecular docking, and COX-1,2/-5-LOX inhibition profile of some novel quinoline derivatives. Bioorg Chem 2018-03-20 [PMID: 29602046]