Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)

Images

 
Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) [KA1329] - Inhibition of soybean 15-lipoxygenase by NDGA (IC50= 9 uM).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, Enzyme Activity
Suitable Sample Type
Compounds expected to inhibit Lipoxygenases

Order Details

Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) Summary

Description
Lipoxygenase Inhibitor Screening Assay Kit is a measurement of detecting hydeoperoxides produced in the lipoxygenation reaction.
Assay Type
Colorimetric
Sample Volume
10 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Assay Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • Functional
Application Notes
Useful in Inhibition assay and Screening.
Publications
Read Publications using KA1329.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 15-Lipoxygenase Standard
  2. 96-Well Cover Sheet
  3. 96-Well Solid Plate (Colorimetric Assay)
  4. Arachidonic Acid (Substrate)
  5. Developing Reagent 1
  6. Developing Reagent 2
  7. Linoleic Acid (Substrate)
  8. Lipoxygenase Inhibitor Screening Assay Buffer (10X)
  9. Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor
  10. Potassium Hydroxide

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Lipoxygenases (LOs) are non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system. The initial product of this reaction is a 4-hydroperoxy cis-trans-1,3-conjugated pentadienyl moiety within the unsaturated fatty acid. The three main LO enzymes are designated 5-, 12-, and 15-LO based on the position of the introduced hydroperoxide. Linoleate and arachidonate are the common substrates for LOs in plants and animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lipoxygenase Inhibitor Screening Kit (KA1329)(5)

Reviews for Lipoxygenase Inhibitor Screening Kit (KA1329) (0)

There are no reviews for Lipoxygenase Inhibitor Screening Kit (KA1329). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Lipoxygenase Inhibitor Screening Kit (KA1329) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Lipoxygenase Inhibitor Screening Products

Blogs on Lipoxygenase Inhibitor Screening

There are no specific blogs for Lipoxygenase Inhibitor Screening, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) and receive a gift card or discount.