Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric)


Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) [KA1329] - Inhibition of soybean 15-lipoxygenase by NDGA (IC50= 9 uM).

Product Details

Reactivity HuSpecies Glossary
Applications Func, Enzyme Activity
Suitable Sample Type
Compounds expected to inhibit Lipoxygenases

Order Details

Lipoxygenase Inhibitor Screening Assay Kit (Colorimetric) Summary

Lipoxygenase Inhibitor Screening Assay Kit is a measurement of detecting hydeoperoxides produced in the lipoxygenation reaction.
Assay Type
Sample Volume
10 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Assay Kit (Colorimetric)


  • Enzyme Activity
  • Functional
Application Notes
Useful in Inhibition assay and Screening.
Read Publications using KA1329.

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 15-Lipoxygenase Standard
  2. 96-Well Cover Sheet
  3. 96-Well Solid Plate (Colorimetric Assay)
  4. Arachidonic Acid (Substrate)
  5. Developing Reagent 1
  6. Developing Reagent 2
  7. Linoleic Acid (Substrate)
  8. Lipoxygenase Inhibitor Screening Assay Buffer (10X)
  9. Nordihydroguaiaretic Acid (NDGA) Positive Control Inhibitor
  10. Potassium Hydroxide


This product is produced by and distributed for Abnova, a company based in Taiwan.


Lipoxygenases (LOs) are non-heme iron-containing dioxygenases that catalyze the addition of molecular oxygen to fatty acids containing a cis,cis-1,4-pentadiene system. The initial product of this reaction is a 4-hydroperoxy cis-trans-1,3-conjugated pentadienyl moiety within the unsaturated fatty acid. The three main LO enzymes are designated 5-, 12-, and 15-LO based on the position of the introduced hydroperoxide. Linoleate and arachidonate are the common substrates for LOs in plants and animals.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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