Lipid A LPS Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Lipid A LPS Antibody [NB100-64484] - IF assay using NB100-64484 at a 1:100 dilution to stain Shigella Sonnei using above protocol. The magnification is 400X.

• Reactivity: Ba
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 4 mg/ml

Lipid A LPS Antibody - BSA Free Summary

• Immunogen: Whole cell preparation of lipid A from E. coli O157.
• Specificity: NB100-64484 recognizes the Lipid A or endotoxin region of LPS (Lipopolysaccharide) from E. coli. Lipid A is a glucosamine-based phospholipid which forms part of the bacterial outer membrane and anchors LPS to the surface of the bacteria. This antibody cross reacts with P. aeruginosa, K. pneumoniae, S. enteriditis, E. aerogenes, E. hermanii, Y. enterocolitica and S. sonnei.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:34199851)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 4 mg/ml
• Purity: IgG purified

Alternate Names for Lipid A LPS Antibody - BSA Free

• Lipid A Lipopolysaccharide

Background

Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipid A LPS Antibody (NB100-64484)(1)

Publication using NB100-64484

• Zaruba M, Roschitz L, Sami H et al. Surface Modification of E. coli Outer Membrane Vesicles with Glycosylphosphatidylinositol-Anchored Proteins: Generating Pro/Eukaryote Chimera Constructs Membranes 2021-06-04 [PMID: 34199851] (WB)