![Western Blot: Lipid A Antibody [NB600-1505] - Effect of nitrogen afterglow exposure on lipid. Dot blots of lipid A pure (left panel) and present in E. coli extracts (right picture); see experiment description in the Method section. The results are expressed as % of residual lipid A vs the vacuum-treated control. On the right panel, determination of the lipid A content in exposed bacteria. Dot blot results were analyzed with the dot calibration curve and relative quantity of bacteria lipid A estimated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116083) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipid-A-Antibody-Western-Blot-NB600-1505-img0001.jpg "Western Blot: Lipid A Antibody [NB600-1505] - Effect of nitrogen afterglow exposure on lipid. Dot blots of lipid A pure (left panel) and present in E. coli extracts (right picture); see experiment description in the Method section. The results are expressed as % of residual lipid A vs the vacuum-treated control. On the right panel, determination of the lipid A content in exposed bacteria. Dot blot results were analyzed with the dot calibration curve and relative quantity of bacteria lipid A estimated. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116083) licensed under a CC-BY license.")
| Immunogen | Whole cell prep of Lipid A from E. coli O157. |
| Localization | Secreted |
| Specificity | Lipid A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Ion exchange chromatography |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Purity is > 95% by sodium sulfate precipitation and ion-exchange chromatography. Use in Dot blot reported in scientific literature (PMID 25837580). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.2) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 4.5 mg/ml |
| Purity | Ion exchange chromatography |
| Publication using NB600-1505 | Applications | Species |
|---|---|---|
| Zerrouki H, Rizzati V, Bernis C et al. Escherichia coli Morphological Changes and Lipid A Removal Induced by Reduced Pressure Nitrogen Afterglow Exposure PLoS One 2015-04-03 [PMID: 25837580] (Cytometric Bead Assay Standard, E. coli) | Cytometric Bead Assay Standard | E. coli |
Secondary Antibodies |
Isotype Controls |
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![Dot Blot: Lipid A Antibody [NB600-1505] - Effect of nitrogen afterglow exposure on lipid. Dot blot binding assay: Increasing concentrations of lipid A were spotted on nitrocellulose membranes and blotted with an anti lipid A antibody. The relative intensity of each spot was quantified (Image J), allowing to build a dose-response calibration curve. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116083) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipid-A-Antibody-Dot-Blot-NB600-1505-img0002.jpg "Dot Blot: Lipid A Antibody [NB600-1505] - Effect of nitrogen afterglow exposure on lipid. Dot blot binding assay: Increasing concentrations of lipid A were spotted on nitrocellulose membranes and blotted with an anti lipid A antibody. The relative intensity of each spot was quantified (Image J), allowing to build a dose-response calibration curve. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0116083) licensed under a CC-BY license.")