Lightning-Link® Rapid Type B Biotin Antibody Labeling Kit

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Product Details

Summary
Conjugate
Biotin
Format
Biotin (A=N/A, E=N/A)
Kit Type
Antibody Labeling Kit

Order Details

Lightning-Link® Rapid Type B Biotin Antibody Labeling Kit Summary

Description
Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (See protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes).

Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.

Features Benefits
Quick and easy to use Save time, no special knowledge required
No separation steps 100% recovery - no antibody/protein loss
Can be used in a wide range of applications Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more) Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 references Successfully used in many fields of research


The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background.

Lightning-Link Biotin Has been optimized for two separate applications:
  • Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used.
  • Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
Kit Type
Antibody Labeling Kit

Applications/Dilutions

Application Notes
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 20 ug of antibody.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Lightning-Link Rapid mix
  2. 1 vial of LL-Rapid Quencher reagent
  3. 1 vial of LL-Rapid Modifier reagent

Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

This product is manufactured by Expedeon Inc. and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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FAQs for Lightning-Link (R) Rapid Type B Biotin Kit (371-0030). (Showing 1 - 3 of 3 FAQ).

  1. Hi, I'm interested in buying your Lightning-Link Biotin labelling kit. I would like to couple biotin to an antibody, which would bind to an antigen in a sandwich assay format. Then streptavidin-coated nanoparticles would be used as the detection scheme. Which type is best for this purpose, type A or type B? Since Type A is for when Strep is used as a detection reagent, but Type B says its best for when Strep is immobilised to the suface of nanoparticles. In my case it is both.
    • Type B would be the preferred kit.
  2. Whats the difference between Lightning-Link (R) Rapid Type A and Type B for Biotin antibody labeling?
    • Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used.Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
  3. I have been using your Lightning Link Biotin kit Type B and liked to know the range of the molar ratio of biotin to IgG (or protein) under your recommended condition and concentration.
    • The molar ratio is 1-3 molecule of biotin for every molecule of IgG.

Other Available Formats

Biotin 371-0030

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